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On sampling and sampling errors in histomorphometry of peripheral nerve fibers

✍ Scribed by Stefano Geuna; Davilene Gigo-Benato; Antonio De Castro Rodrigues

Publisher
John Wiley and Sons
Year
2004
Tongue
English
Weight
200 KB
Volume
24
Category
Article
ISSN
0738-1085

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✦ Synopsis

Abstract

Histomorphometrical assessment of regenerated peripheral nerves is a very common goal of many studies in experimental microsurgery. In this paper, the main critical issues in nerve fiber sampling for quantitative morphological assessment are addressed. The equal opportunity rule, i.e., the basic paradigm of random sampling, is described, together with an explanation of how sampling errors, in the selection of histologic fields and of the nerve fibers inside them, can produce a bias in quantitative estimates. Finally, some practical suggestions on how to cope with the most common sampling errors are provided, in order to help researchers obtain reliable histomorphometrical data on peripheral nerve fibers. Β© 2003 Wiley‐Liss, Inc.

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