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On optimizing the blocking step of indirect enzyme-linked immunosorbent assay for Epstein-Barr virus serology

✍ Scribed by Chun Shen Lim; Gopala Krishnan; Choon Kook Sam; Ching Ching Ng

Book ID
118427837
Publisher
Elsevier Science
Year
2013
Tongue
English
Weight
324 KB
Volume
415
Category
Article
ISSN
0009-8981

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Development of an enzyme-linked immunoso
Development of an enzyme-linked immunosorbent assay (ELISA) for detecting IgA antibodies to the epstein-barr virus
✍ Wu-Ching Uen; Janos Luka; Gary R. Pearson πŸ“‚ Article πŸ“… 1988 πŸ› John Wiley and Sons 🌐 French βš– 506 KB

A 3-step enzyme-linked immunosorbent assay (ELISA) was developed for detecting IgA antibodies to purified Epstein-Barr virus (EBV) polypeptides. The 3-step procedure included the use of a mouse anti-human IgA monoclonal antibody (MAb) to amplify the IgA reaction. The 2 major EBV proteins used in thi