On-line solid-phase extraction in microcolumn- liquid chromatography coupled to UV or MS detection: Application to the analysis of cyanobacterial toxins
✍ Scribed by C. Rivasseau; G. Vanhoenacker; P. Sandra; M.-C. Hennion
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 155 KB
- Volume
- 12
- Category
- Article
- ISSN
- 1040-7685
No coin nor oath required. For personal study only.
✦ Synopsis
An on-line technique coupling preconcentration via a microprecolumn Ž . with microcolumn-liquid chromatography micro-LC on a 1-mm inner diameter Ž .
i.d. analytical column was investigated. The system was found to be very simple, fast, and efficient for the trace-level determination of some natural hepatotoxins synthesized by Cyanobacteria, namely, microcystins, in environmental water. Particular attention was paid to band broadening, and a good quality coupling was Ž . obtained. A cleanup was introduced in the solid-phase extraction SPE sequence to eliminate part of the interferents co-extracted during the sample percolation. The technique was reproducible, with relative standard deviations ranging from 3 to 8%, linear in the range 0.05᎐50 grL. Microcystins could be detected below the 0.04 grL level in drinking and surface water using only 5-mL sample volumes, with UV detection. The very small sample volume, resulting from lower detection limits, in absolute amounts, obtained in micro-LC represents a definite advantage over on-line classical chromatography. The suitability of on-line micro-SPE᎐micro-LC᎐MS was also evaluated. Application to the determination of microcystins in environmental and drinking water samples are presented.