On-line radiometric determination of [14C]-urapidil and its main metabolises in rat plasma, using post-column ion-pair extraction and solvent segmentation techniques
✍ Scribed by A.C. Veltkamp; H.A. Das; R.W. Frei; U.A.Th. Brinkman
- Book ID
- 103910996
- Publisher
- Elsevier Science
- Year
- 1988
- Tongue
- English
- Weight
- 1000 KB
- Volume
- 6
- Category
- Article
- ISSN
- 0731-7085
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✦ Synopsis
Post-column ion-pair extraction has been applied to the on-line radiometric determination of [14C]-urapidil and its main metabolites in reversed-phase liquid chromatography (LC). ['"Cl-remoxipride was used as internal standard. Chromatography was performed isocratically, using acetonitrile-water (pH = 2.2) as eluent on a cyanobonded column. Various ion-pair reagents were tested for their extraction efficiency of the analytes mentioned from the eluent into chloroform. Quantitative extraction was possible using sodium dodecylbenzenesulphonate as ion-pair reagent. The influence of the extraction solvent composition, the percentage organic modifier in the eluent and the flow-rates of eluent and scintillator on the extraction percentage and the 14C-counting efficiency has been studied. In addition, the enhancement of sensitivity is possible using an on-line radiometric procedure. Finally, the method has been applied to the analysis of rat plasma for ['4C]-labelled urapidil and its metabolites.