On-line preparation of peroxymonocarbonate and its application for the study of energy transfer chemiluminescence to lanthanide inorganic coordinate complexes
✍ Scribed by Meilin Liu; Xianglei Cheng; Lixia Zhao; Jin-Ming Lin
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 194 KB
- Volume
- 21
- Category
- Article
- ISSN
- 1522-7235
- DOI
- 10.1002/bio.903
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✦ Synopsis
Abstract
It has been shown that peroxymonocarbonate ion (HCO~4~^−^) is a potent oxidant. In this study, a flow‐injection system was developed in order to prepare on‐line HCO~4~^−^ ion and the optimum conditions for the on‐line preparation of HCO~4~^−^ were studied in detail. We used 99% ^13^C‐enriched NaHCO~3~ to examine peroxymonocarbonate by ^13^C‐NMR at 25°C. An ultra‐weak chemiluminescence (CL) was observed after mixing H~2~O~2~ and sodium bicarbonate in an organic co‐solvent that can accelerate the formation of HCO~4~^−^ ion. When lanthanide inorganic coordinate complex, Eu(II)–EDTA, was added into this HCO~4~^−^ system, the CL intensity was significantly enhanced. The CL mechanism was investigated by various methods. The experimental results indicate that peroxymonocarbonate oxidizes Eu(II) to Eu(III) and produces singlet oxygen; meanwhile, the energy originating from dimers of singlet oxygen is accepted by the Eu(III)–EDTA^−^ complex. The excited Eu(III) ions undergo radiative deactivation and emit CL. Copyright © 2006 John Wiley & Sons, Ltd.