On-line high-performance liquid chromatography/mass spectrometric investigation of amyloid-β peptide variants found in Alzheimer's disease

✍ Scribed by Andrew J. Thompson; Teck K. Lim; Colin J. Barrow

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 57 KB
• Volume: 13
• Category: Article
• ISSN: 0951-4198
• DOI: 10.1002/(sici)1097-0231(19991215)13:23<2348::aid-rcm797>3.0.co;2-j

✦ Synopsis

Ab peptides are the major components of amyloid deposits in Alzheimer's disease. The presence of Nterminally truncated Ab variants in amyloid may be a critical factor in Alzheimer's disease pathogenesis. These Ab variants are less soluble and more amyloidogenic than full-length Ab, making their separation, purification and identification difficult. High-performance liquid chromatography (HPLC) at elevated temperatures, coupled to electrospray ionization (ES) mass spectrometry (MS), enables rapid separation and identification of N-terminally truncated Ab variants. This methodology provides a potential tool for exploring the importance of these Ab variants in both the pathogenesis and diagnosis of Alzheimer's disease.