On-line electrochemical derivatization combined with diode-array detection in flow-injection analysis : Rapid Determination of Etoposide and Teniposide in Blood Plasma

✍ Scribed by M.A.J. Van Opstal; J.S. Blauw; J.J.M. Holthuis; W.P. Van Bennekom; A. Bult

• Publisher: Elsevier Science
• Year: 1987
• Tongue: English
• Weight: 809 KB
• Volume: 202
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/s0003-2670(00)85900-0

✦ Synopsis

On-line electrochemical derivatization combined with diode-array u.v./visible detection in flowinjection systems greatly enhances the selectivity. The system is applied for the determination of the antineoplastic agents etoposide (VP 16-213) and teniposide (VM 26) in blood plasma. The optimum oxidation potential, detection wavelength, solvent composition, pH and flow rate are established. Calibration graphs for spiked plasma are linear in the range l-50 ,ug ml-' for both etoposide and teniposide. The detection limit in absolute amount is 6 ng for each compound at a signal-to-noise ratio of 3. The injection frequency can be as high as 40 h-'. The method is validated by quantifying teniposide in plasma of a patient treated with teniposide.