On-line determination of animal cell concentration

✍ Scribed by P. Ducommun; I. Bolzonella; M. Rhiel; P. Pugeaud; U. von Stockar; I. W. Marison

• Publisher: John Wiley and Sons
• Year: 2001
• Tongue: English
• Weight: 148 KB
• Volume: 72
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/1097-0290(20010305)72:5<515::aid-bit1015>3.0.co;2-q

✦ Synopsis

A new approach for the indirect determination of cell concentration in the case of nonconstant metabolic rates has been developed. The specific glucoseuptake rate was shown to be nonconstant in batch cultures of free suspended and immobilized CHO SSF3 cells. Time-independent models correlating the specific rate to the limiting substrate concentration were established, thus providing a continuous determination of the specific rate through on-line measurement of the limiting substrate. The method could be applied to determine online cell concentration in both free suspended and immobilized cell cultures. Results were verified off-line by crystal violet nuclei counting. The predicted cell concentration was in very good agreement with the off-line reference during the whole exponential-growth phase, until the specific glucose-uptake rate tended to zero.