On-line coupling of isolation/in situ concentration integrated with derivative synchronous spectrofluorimetry for the simultaneous determination of epinephrine and norepinephrine in urine

✍ Scribed by P. Cañizares; M.D.Luque de Castro

• Publisher: Elsevier Science
• Year: 1995
• Tongue: English
• Weight: 620 KB
• Volume: 317
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(95)00421-1

✦ Synopsis

An unsegmented flow system for the automated determination of epinephrine and norepinephrine in urine is proposed. The simultaneous determination involves the following on-line steps: (i) matrix isolation/analyte preconcentration in an exchange minicolumn located in the loop of a injection valve; (ii) elution-injection into the dynamic system and derivatization during the transport to the detector; (iii) in situ concentration in the flow cell integrated with the synchronous spectrofluorimetric scan; (iv) data acquisition and derivation by computer. The method thus developed enables a linear determination range between 2 and 60 ng ml-' for both catecholamines with R.S.D.s of 1.0 and 2.0% for norepinephrine and epinephrine, respectively, and a sample throughput of 12 samples h-'. The utility of the method has been proved by applying it to urine samples with low and high concentrations of the target analytes.