Oxygen consumption rates were measured in a respirometer for different mammalian cell lines (BHK, murine hybridoma, and CHO), and the effects of cell density (1-20 million cells/mL) and temperature (6 to 37ยฐC) on specific oxygen consumption rate were investigated. The specific oxygen consumption was
โฆ LIBER โฆ
On-line continuous measurement of the oxygen consumption rate in mammalian cell culture
โ Scribed by Mutsumi Takagi; Kazuo Ueda
- Book ID
- 113397290
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 253 KB
- Volume
- 77
- Category
- Article
- ISSN
- 0922-338X
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Measurement of oxygen uptake rate is useful in assessing growth, viability, and metabolic activity. In cell culture, however, the oxygen demand is extremely small (typically 0.1-0.3 mMOJL-h) and is very difficult to measure accurately using conventional offgas analysis. In many industrial submerged