On-line capillary electrophoresis-electrospray ionization mass spectrometry using a polymerized anionic surfactant

✍ Scribed by Wenzhe Lu; Shahab A. Shamsi; Tracy D. McCarley; Professor Isiah M. Warner

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 582 KB
• Volume: 19
• Category: Article
• ISSN: 0173-0835
• DOI: 10.1002/elps.1150191225

✦ Synopsis

Abstract

On‐line capillary electrophoresis ‐ electrospray ionization ‐ mass spectrometry (CE‐ESI‐MS) has been used to determine the tricyclic antidepressant drugs (imipramine, doxepin, and amitriptyline) as well as the β‐adrenergic blocker drugs (propranolol and alprenolol). A CE‐ESI‐MS interface linking a manually operated CE system and a Finnigan MAT‐900 sector mass spectrometer (with an Analytica electrospray ionization source) has been constructed in‐house and employed for this study. Although a water/methanol based capillary zone electrophoresis (CZE) buffer was initially used to determine these analytes, enhanced resolution was obtained by addition of a polymerized surfactant, i.e., poly‐sodium undecylenic sulfate (poly‐SUS), into the electrokinetic chromatography (EKC) buffer. When a low concentration of this poly‐SUS surfactant was added to a volatile EKC buffer, these structurally similar cationic drugs were EKC separated and on‐line detected by ESI‐MS.