On-column sample enrichment for the high-sensitivity sheath-flow CE-MS analysis of peptides

Sample purification and preparation tech

Sample purification and preparation technique based on nano-scale reversed-phase columns for the sensitive analysis of complex peptide mixtures by matrix-assisted laser desorption/ionization mass spectrometry

✍ Gobom, Johan; Nordhoff, Eckhard; Mirgorodskaya, Ekaterina; Ekman, Rolf; Roepstor 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 839 KB

A simple reversed-phase nano-column purification and sample preparation technique is described, which markedly improves the mass spectrometric analysis of complex and contaminated peptide mixtures by matrix-assisted laser desorption/ionization (MALDI). The method is simple, fast and utilizes only lo