On a water soluble factor neutralizing antibodies against the primary acceptor in photosynthetic electron transport of chloroplasts
✍ Scribed by G. Regitz; R. Berzborn; A. Trebst
- Book ID
- 104751814
- Publisher
- Springer-Verlag
- Year
- 1970
- Tongue
- English
- Weight
- 518 KB
- Volume
- 91
- Category
- Article
- ISSN
- 0032-0935
No coin nor oath required. For personal study only.
✦ Synopsis
Rabbits immunized against the thylakoid system of chloroplasts form two different antibodies against the reducing site of photosystem I. One inhibits photosynthetic NADP+ and fcrredoxin dependent cytochrome c reduction, but not photosynthetic anthraquinone reduction by isolated spinach chloroplasts. The other inhibits all three reductions. Both do not inhibit a Hill reaction with ferrieyanide. The presumed antigen moiety against these antibodies was isolated as a water soluble factor released from lyophilized chloroplasts after treatment with diethylether. The heat stable, nondialysable factor has absorption peaks at 262 and 315 m~. It is autoxidizable and has the property of a cytochrome c reducing substance. The factor neutralizes the antibody inhibition of photosynthetic anthraqninone and ferredoxin reduction. It is proposed that the soluble factor is the prosthetic group of the primary accepter of photosystem I.