Oligovalent Fab Display on M13 Phage Improved by Directed Evolution

Protein trans-splicing on an M13 bacteri

Protein trans-splicing on an M13 bacteriophage: towards directed evolution of a semisynthetic split intein by phage display

✍ Daniel Garbe; Ilka V. Thiel; Henning D. Mootz 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 278 KB

## Abstract Split inteins link their fused peptide or protein sequences with a peptide bond in an autocatalytic reaction called protein __trans__‐splicing. This reaction is becoming increasingly important for a variety of applications in protein semisynthesis, polypeptide circularisation, construct