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Oligonucleotide duplexes containing CC(A/T)GG stimulate cleavage of refractory DNA by restriction endonuclease EcoRII

✍ Scribed by Claus-Dietmar Pein; Monika Reuter; Dieter Cech; Detlev H. Krüger


Book ID
115921571
Publisher
Elsevier Science
Year
1989
Tongue
English
Weight
377 KB
Volume
245
Category
Article
ISSN
0014-5793

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Use of Specific Oligonucleotide Duplexes
✍ M. Reuter; D. Kupper; C.D. Pein; M. Petrusyte; V. Siksnys; B. Frey; D.H. Kruger 📂 Article 📅 1993 🏛 Elsevier Science 🌐 English ⚖ 470 KB

There are numerous restriction endonucleases (ENases) which are known never to achieve total cleavage of certain unmethylated target DNAs. In addition to EcoRII we found seven ENases (AtuBI, Cfr9I, Eco57I, Ksp632I, NaeI, NarI, and SauBMKI) that were stimulated by oligodeoxyribonucleotide (oligo) dup