Oligonucleotide-conjugated Thiazole Orange Probes as “Light-up” Probes for Messenger Ribonucleic Acid Molecules in Living Cells

Light-Up Probes: Thiazole Orange-Conjuga

Light-Up Probes: Thiazole Orange-Conjugated Peptide Nucleic Acid for Detection of Target Nucleic Acid in Homogeneous Solution

✍ Nicke Svanvik; Gunnar Westman; Dongyuan Wang; Mikael Kubista 📂 Article 📅 2000 🏛 Elsevier Science 🌐 English ⚖ 138 KB

We have constructed light-up probes for nucleic acid detection. The light-up probe is a peptide nucleic acid (PNA) oligonucleotide to which the asymmetric cyanine dye thiazole orange (TO) is tethered. It combines the excellent hybridization properties of PNA and the large fluorescence enhancement of