Oligonucleotide-arrayed TFT photosensor applicable for DNA chip technology
✍ Scribed by Tsuyoshi Tanaka; Keiichi Hatakeyama; Masahiro Sawaguchi; Akihito Iwadate; Yasushi Mizutani; Kazuhiro Sasaki; Naofumi Tateishi; Haruko Takeyama; Tadashi Matsunaga
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 194 KB
- Volume
- 95
- Category
- Article
- ISSN
- 0006-3592
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✦ Synopsis
Abstract
A thin film transistor (TFT) photosensor fabricated by semiconductor integrated circuit (IC) technology was applied to DNA chip technology. The surface of the TFT photosensor was coated with TiO~2~ using a vapor deposition technique for the fabrication of optical filters. The immobilization of thiolated oligonucleotide probes onto a TiO~2~‐coated TFT photosensor using γ‐aminopropyltriethoxysilane (APTES) and N‐(γ‐maleimidobutyloxy) sulfosuccinimide ester (GMBS) was optimized. The coverage value of immobilized oligonucleotides reached a plateau at 33.7 pmol/cm^2^, which was similar to a previous analysis using radioisotope‐labeled oligonucleotides. The lowest detection limits were 0.05 pmol/cm^2^ for quantum dot and 2.1 pmol/cm^2^ for Alexa Fluor 350. Furthermore, single nucleotide polymorphism (SNP) detection was examined using the oligonucleotide‐arrayed TFT photosensor. A SNP present in the aldehyde dehydrogenase 2 (ALDH2) gene was used as a target. The SNPs in ALDH2*1 and ALDH2*2 target DNA were detected successfully using the TFT photosensor. DNA hybridization in the presence of both ALDH2*1 and ALDH2*2 target DNA was observed using both ALDH2*1 and ALDH2*2 detection oligonucleotides‐arrayed TFT photosensor. Use of the TFT photosensor will allow the development of a disposable photodetecting device for DNA chip systems. © 2006 Wiley Periodicals, Inc.