Oligodendrocytes of the jimpy phenotype can be partially restored by environmental factors in vivo
✍ Scribed by Dr. F. Lachapelle; P. Lapie; A. T. Campagnoni; M. Gumpel
- Publisher
- John Wiley and Sons
- Year
- 1991
- Tongue
- English
- Weight
- 904 KB
- Volume
- 29
- Category
- Article
- ISSN
- 0360-4012
No coin nor oath required. For personal study only.
✦ Synopsis
Cross-transplantations of neural tissue between jimpy up) shiverer (shi) and normal mice have been performed under heterochronic conditions. In all series, fragments of E14-El5 embryonic neural tissue from the different donors have been transplanted into newborn host brain in order to study environmental influences by differentiated tissue on transplanted embryonic cell lines. Large patches of proteolipid protein (PLP)-positive myelin have been observed in the j p brain after transplantation of shi or normal embryonic tissue into the newborn j p brain, suggesting that the j p parenchyma did not inhibit the differentiation of other oligodendrocytes (ODCs). Jp embryonic tissue had the same mitotic potential as normal tissue, as demonstrated by the larger size of myelin patches observed when j p embryonic tissue was used instead of newborn j p tissue. By contrast, whatever the conditions, jp myelin patches were always obviously smaller than normal or shi myelin patches, suggesting that the myelinating capacity of j p ODCs was not enhanced by environmental factors. Finally, comparison of the ratio of successful outcomes observed following embryonic vs. newborn j p donor tissue, strongly suggests a partial or total normalization of j p embryonic ODCs survival by a more mature shi environment.