Oil-frozen W1/O/W2 double emulsions for dermal biomacromolecular delivery containing ethanol as chemical penetration enhancer
✍ Scribed by Yuly A. Jaimes-Lizcano; Louise B. Lawson; Kyriakos D. Papadopoulos
- Book ID
- 102402629
- Publisher
- John Wiley and Sons
- Year
- 2011
- Tongue
- English
- Weight
- 434 KB
- Volume
- 100
- Category
- Article
- ISSN
- 0022-3549
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✦ Synopsis
Oil-frozen water-in-oil-in-water (W 1 /O/W 2 ) double emulsions (DE) containing ethanol up to 40% (w/v) in the external aqueous W 2 phase exhibited external coalescence upon thawing of the oil phase, releasing up to 85% of the encapsulated protein of the internal aqueous phase. These emulsions were studied in vitro as potential dermal macromolecular delivery formulations, achieving fluorescein isothiocyanate-labeled bovine serum albumin (FITC-BSA) penetration of up to 86 :m into porcine skin, reaching the viable epidermis where the immunocompetent Langerhans cells are located. Enzyme-linked immunosorbent assay was performed to observe the effect of the emulsification process and ethanol content on the ability of BSA to form antigen-antibody complexes; results indicated that ethanol content and the emulsification process did not diminish the BSA-antibody complex formation when compared with a BSA standard aqueous solution. Therefore, it is shown that oil-frozen W 1 /O/W 2 DE, with penetration-enhancing ethanol in the W 2 phase, can potentially be used for cutaneous vaccine delivery formulations.