𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Off-line capillary electrophoresis/fully automated nanoelectrospray chip quadrupole time-of-flight mass spectrometry and tandem mass spectrometry for glycoconjugate analysis

✍ Scribed by Laura Bindila; Reinaldo Almeida; Alistair Sterling; Mark Allen; Jasna Peter-Katalinić; Alina Zamfir

Publisher
John Wiley and Sons
Year
2004
Tongue
English
Weight
803 KB
Volume
39
Category
Article
ISSN
1076-5174

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Implementation and optimization of an off‐line capillary electrophoresis (CE)/(−)nanoESIchip‐quadrupole time‐of‐flight (QTOF) mass spectrometric (MS) and tandem MS system for compositional mapping and structural investigation of components in complex carbohydrate mixtures is described. The approach was developed for glycoscreening and applied to O‐glycosylated peptides from urine of a patient suffering from α‐N‐acetylhexosaminidase deficiency, known as Schindler's disease. The fundamental issue of sensitivity, previously representing a serious drawback of the off‐line CE/MS analysis, could be positively addressed by the off‐line conjunction of CE with automated chip‐based ESI‐QTOF‐MS to provide flexibility for CE/chip MS coupling and enhance structural elucidation of single components in heterogeneous mixtures. Copyright © 2004 John Wiley & Sons, Ltd.

📜 SIMILAR VOLUMES

On-line sheathless capillary electrophor
On-line sheathless capillary electrophoresis/nanoelectrospray ionization-tandem mass spectrometry for the analysis of glycosaminoglycan oligosaccharides
✍ Alina Zamfir; Daniela G. Seidler; Elke Schönherr; Hans Kresse; Jasna Peter-Katal 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 English ⚖ 220 KB

## Abstract A novel approach in glycosaminoglycomics, based on sheathless on‐line capillary electrophoresis/nanoelectrospray ionization‐quadrupole time of flight‐mass spectrometry (CE/nanoESI‐QTOF‐MS) and tandem MS of extended chondroitin sulfate/dermatan (CS/DS) oligosaccharide chains is described