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Occurrence of O-phosphorylated 2-keto-3-deoxyoctonate in the lipopolysaccharide of Bacteroides gingivalis

✍ Scribed by Hidefumi Kumada; Kiyoko Watanabe; Toshio Umemoto; Yuji Haishima; Seiichi Kondo; Kazuhito Hisatsune


Book ID
109314466
Publisher
John Wiley and Sons
Year
1988
Tongue
English
Weight
242 KB
Volume
51
Category
Article
ISSN
0378-1097

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A procedure is described to determine 2-keto-3-deoxyoctonate (KDO) present in lipopolysaccharide (LPS) of gram-negative bacteria. The method involves the treatment of LPS with 0.2 N H,SO, at 100Β°C for 30 min to release KDO, followed by its reaction with periodic acid, sodium arsenite, and thiobarbit