Occurrence of nuclear βII-tubulin in cultured cells
✍ Scribed by Consuelo Walss-Bass; Keliang Xu; Sebastien David; Arlette Fellous; Richard F. Ludueña
- Book ID
- 106138432
- Publisher
- Springer-Verlag
- Year
- 2002
- Tongue
- English
- Weight
- 637 KB
- Volume
- 308
- Category
- Article
- ISSN
- 0302-766X
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The relative amount of free and microtubule-associated tubulin in tissue culture cells was determined by coichicine binding. Both microtubules and tubulin were stabilized in a dilute homogenate containing 50% glycerol and 5% dimethylsu~oxide. Microtubules were separated by sedimentation at lOO,t@Og
Microtubules and actin filaments are two of the major components of the cytoskeleton. There is accumulating evidence for interaction between the two networks. Both the ␣and -subunits of tubulin exist as numerous isotypes, some of which have been highly conserved in evolution. In an effort to better