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Occupied and unoccupied type II estrogen binding sites in human breast cancer

✍ Scribed by M.T.P. Lopes; M.H. Liberato; A. Widman; M.M. Brentani

Book ID
116006153
Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
966 KB
Volume
26
Category
Article
ISSN
0022-4731

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## Abstract We show that flavonoids positively regulate type‐II estrogen‐binding site (type‐ll EBS) levels both in MCF‐7 (ER‐positive) and in MDA‐MB231 (ER‐negative) breast‐cancer cells. Type‐ll EBS were measured by a whole‐cell assay at 4°C for 2.5 hr using [^3^H]‐estradiol as tracer. In both cell