Observer-based non-linear control of a fed-batch autoinductive fermentation process

✍ Scribed by B. Tartakovsky; B.E. Dainson; D.R. Lewin; M. Sheintuch

• Publisher: Elsevier Science
• Year: 1996
• Tongue: English
• Weight: 798 KB
• Volume: 61
• Category: Article
• ISSN: 0923-0467
• DOI: 10.1016/0923-0467(96)80021-2

✦ Synopsis

Optimal regulatory control of an autoinductive recombinant culture in a fed-batch reactor is considered. End point optimization results in a three-stage process: biomass growth, inducer synthesis and product synthesis. It is shown that in the last stage the substrate concentration should be maintained constant. This is achieved using an input-output linearizing controller accompanied by a novel non-linear state observer for the estimation of unmeasured state variables on the basis of on-line off-gas carbon dioxide concentration measurements. Experimental runs of luminous recombinant (E). coli strain in a laboratory fermenter demonstrate the rapid convergence of the observer estimates as well as the effectiveness and robustness of the overall control system.