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Observations on the effects of low frequency electromagnetic fields on cellular transcription in Drosophila larvae reared in field-free conditions

✍ Scribed by David R. Tipping; Karen E. Chapman; Andrew J. Birley; Moray Anderson

Publisher: John Wiley and Sons
Year: 1999
Tongue: English
Weight: 38 KB
Volume: 20
Category: Article
ISSN: 0197-8462
DOI: 10.1002/(sici)1521-186x(1999)20:2<129::aid-bem8>3.0.co;2-q

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✦ Synopsis

Drosophila larvae reared inside a m-metal box with an internal field strength 0.004 mT, were treated with a magnetic field of 50 Hz, 8 mT, for 20 min. Control experienced 0.004 mT. Cellular transcript levels were assessed using slot blots and quantified using a Phosphorimager. Blots were hybridised using probes against HSP 70a, Histone 1.9, and Copia. The low frequency EMFs very significantly decreased transcript levels, indicating that experimental responses may be influenced by previous exposure or lack of previous exposure.