Observations on specific Giemsa staining of the Y and on selective oil destaining of the chromosomes

✍ Scribed by Bruno Dallapiccola; Nicola Ricci

• Publisher: Springer
• Year: 1975
• Tongue: English
• Weight: 721 KB
• Volume: 26
• Category: Article
• ISSN: 0340-6717
• DOI: 10.1007/bf00281461

✦ Synopsis

The human Y chromosome can be differentially stained with Giemsa using simple procedures. This phenomenon is strikingly to that observed with quinacrine fluorescence. The specific Giemsa-Y stain may be selectively removed by the action of an oil. The same oil, under certain conditions, selectively removes Giemsa stain from all chromosomes, resulting in R- and T-banding patterns. These bands, which are obtained through subtraction of dye from Giemsa-stained chromosomes, allow slides to be further processed.