Observations on enzymes of ammonia assimilation in two different strains ofCyanidium caldarium
✍ Scribed by Carmelo Rigano; Giovanni Aliotta; Vittoria di Martino Rigano
- Publisher
- Springer
- Year
- 1975
- Tongue
- English
- Weight
- 298 KB
- Volume
- 104
- Category
- Article
- ISSN
- 0302-8933
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✦ Synopsis
Two strains of Cyanidium caldarium, one able to utilize nitrate as a substrate, and the other not, were tested for the presence of enzymes of ammonia assimilation. The nitrate-assimilating strain exhibits glutamate dehydrogenase activity. By contrast, the other strain lacks glutamate dehydrogenase; it possesses high alanine dehydrogenase and L-alanine aminotransferase activities which suggest that this strain may incorporate ammonia through reductive amination of pyruvate and may form glutamate from 2-ketoglutarate by a transamination reaction with alanine. Neither strain reveals glutamate synthase activity. Both strains contain similar levels of glutamine synthetase.