Observations on Comparative Growth Studies Between a Streptomycin-Resistant and Streptomycin-Sensitive Strain of Salmonella schottmuelleri**Received August 18, 1959 from the Department of Microbiology and Public Health, Michigan State University, East Lansing.
✍ Scribed by Simmons, Richard J. ;Gainor, Charles
- Publisher
- Elsevier
- Year
- 1960
- Weight
- 346 KB
- Volume
- 49
- Category
- Article
- ISSN
- 0095-9553
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✦ Synopsis
VOLUME 49 FEBRUARY I960 NUMBER 2 Observations on Comparative Growth Studies Between a Streptomycin-Resistant and Streptomycin-Sensitive Strain of Salmonella schottmuelleri * By RICHARD J. SIMMONS and CHARLES GAINOR
Growth comparisons between a streptomycin-resistant and streptomycin-sensitive strain of Salmonella scbottmrelleri is reported. The inability of the resistant strain to utilize ammonium citrate, ammonium succinate, I-asparagine, aspartic acid, and cystine as sole source of nitrogen stands in sharp contrast to the performance of the sensitive strain. The resistant strain failed to grow in a synthetic medium of mineral salts, glucose, and I-asparagine which, however, supported excellent growth of the sensitive strain. Regardless of the nature of the nutritional environments employed in this stud , the resistant strain was never stimulated to a level of growth comparable to d a t of the sensitive strain. Apparently, the rate of utilization of glutamic acid and aspartic acid is the same for each strain, and the two strains produced alanine at approximately the same rate. In biochemical reactions the resistant strain demonstrated a response forty-eight to seventy-two hours later than the sensitive strain, and failed to produce gas from maltose or glucose in seventy-two hours.
In contrast, the sensitive strain gave, in twenty-four hours, reactions comparable to a typical S. scbottmuelleri.
HE PROBLEM of microbial resistance to anti-Tbiotics still requires additional information to explain more fully this perplexing phenomenon. Studies were undertaken to ascertain what differences, if any, exist between a streptomycin-