✦ LIBER ✦

Observation of single-cell fluorescence spectra in laser flow cytometry

✍ Scribed by M. R. Gauci; G. Vesey; J. Narai; D. Veal; K. L. Williams; J. A. Piper

Publisher: John Wiley and Sons
Year: 1996
Tongue: English
Weight: 558 KB
Volume: 25
Category: Article
ISSN: 0196-4763
DOI: 10.1002/(sici)1097-0320(19961201)25:4<388::aid-cyto11>3.0.co;2-r

No coin nor oath required. For personal study only.

✦ Synopsis

We report the first demonstration of directly recording fluorescence spectra of single cells in flow cytometry. A n intensified, 512-element photodiode array was used in conjunction with a dispersing prism to capture the fluorescence emission spectra of Coulter ImmunoCheck calibration beads and Dictyostellum discoideum spores stained with the indocarbocyanine derivative -3, fluorescein isothiocyanate, or R-phycoerythrin. The demonstration was made feasible by enhancing the signal-tonoise ratio of the detection process by using a East gating technique applied to the detector. Results

show that the complete fluorescence spectra of in- dividual stained cells contain information that is normally not captured by conventional flow cytometers. By using the spectrographic flow cytometer, all this information is recorded, allowing small features and shifts in the fluorescence spectra of labelled particles to be studied. o 1996 wuq-Liss, I ~C .

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