Objective identification of cell cycle phases and subphases by automated image analysis
β Scribed by Nicolini, C.; Kendall, F.; Giaretti, W.
- Book ID
- 118517403
- Publisher
- Biophysical Society
- Year
- 1977
- Tongue
- English
- Weight
- 1022 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0006-3495
No coin nor oath required. For personal study only.
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Mouse embryo fibroblasts growing asynchronously in vitro stained with Feulgen method and their nuclear chromatin was analysed by means of the image analysing computer Quantimet 720D. Cells with 2C, 3C and 4C content of DNA were considered as being in G I , middle S and GP phase of cell cycle, respec
## Abstract Budding yeast __Saccharoymyces cerevisiae__ is a powerful model system for analyzing eukaryotic cell cycle regulation. Yeast cell cycle analysis is typically performed by visual analysis or flow cytometry, and both have limitations in the scope and accuracy of data obtained. This study