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O527 - HLA-DP detected by serology

โœ Scribed by Tongio Marie-Marthe; van den Berg Loonen Ella; Bignon Chandanayingyong; Chandanayingyong Dasnayanee; Dormoy Anne; Eiermann Thomas; Marshall William; Park Min Sik; GMTh Schreuder

Book ID
116114933
Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
165 KB
Volume
47
Category
Article
ISSN
0198-8859

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## Detection of HLA-DP serological allodeterminants by the use of radioiodinated DP molecules* HLA class I1 molecules were partially purified from cells of an HLA deletion mutant cell line, LCL721.82, that lost DR and DQ expression but retained DPw2 specificity and labeled with radioactive 1251. T

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A DP serological allospecificity was identified using 'zI-labeled preparations of HLA class I1 molecules isolated from cells of HLA homozygous typing cell lines, SLE (DRw6, DQwl, DPw3) and WT46 (DRw6, DQwl, DPw2), and depleted of DR molecules by absorption with an anti-DR monoclonal antibody. The sp