Introduction: HCV incidence and sero-prevalence among Hungarian donors in colleges and universities was studied. Frequency of potential risk factors for acquiring HCV was determined by questionnaire, which documented whole lifespan history of tattooing, body/ear piercing, acupuncture, dentistry, surgery and accident. Materials and Methods: Screening tests for infectious agents were performed in screening labs. 10 ml native blood sample was taken from each donor for HCV PCR. RNA preparation was made by TNAI kit on COBAS AmpliPrep. Amplification and detection was performed by AmpliScreen HCV v2.0 kit on COBAS Amplicor. Data of 3,041 out of a total 3,160 donors were processed for further statistics. Results: 2,928 donors reported at least one potential exposure to HCV. Tattooing was reported by 7%, acupuncture by 3%, ear piercing by 52%, body piercing by 12%, surgery by 53%, dentistry by 89% and accident by 17%. Most donors (70%) registered 2 or 3 risk factors. Surgery in field of oto-rhino-laryngology was the most frequent (45%). However, proportion of intraabdominal, cardiovascular, oncological and ortopaedic surgery was together 28%. Anti-HCV screening test showed reactive result in 5 samples, however, further confirmation has not verified existence of specific antibodies in these donors. Based on serological results sero-prevalence was 0.0% in the studied population. Based on PCR results there was no evidence for new HCV infection in the studied interval. Odds ratio could not be determined, because HCV infected case was not defined in this study. Conclusions: Sero-prevalence and incidence of HCV in a restricted donor population as well frequency of potential risk factors for acquiring HCV was studied in our pilot study at first in Hungary. Sero-prevalence data presented that Hungary is a moderately infected country, hence role of low-risk factors might be marginal in HCV transmission.