o-phthalaldehyde as a probe in the active site of ribulose 1,5-bisphosphate carboxylase
โ Scribed by A.S. Bhagwat; K.Gopala Krishna
- Book ID
- 113266254
- Publisher
- Elsevier Science
- Year
- 1986
- Tongue
- English
- Weight
- 647 KB
- Volume
- 873
- Category
- Article
- ISSN
- 0167-4838
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
The functions of H i s 291 , H i s 295 and H i s 324 at the active-site of recombinant A. nidulans ribulose-1,5-bisphosphate carboxylase/oxygenase have been explored by site-directed mutagenesis. Replacement of His 291 by K or R resulted in unassembled proteins, while its replacement by E, Q or N re
Mutagenesis in vitro of the gene encoding the large subunit of ribulose-1,5-bisphosphate carboxylase/ oxygenase (EC 4.1.1.39) from Anacystis nidulans was used to generate novel enzymes. Two conserved residues, threonine 4 and lysine 11 in the N-terminus were changed. The substitution of threonine 4