O-2A glial progenitors from mature brain respond to CNS neuronal cell line-derived growth factors
✍ Scribed by S. F. Hunter; J. E. Bottenstein
- Publisher
- John Wiley and Sons
- Year
- 1991
- Tongue
- English
- Weight
- 853 KB
- Volume
- 28
- Category
- Article
- ISSN
- 0360-4012
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✦ Synopsis
Abstract
During development, myelin‐forming oligodendrocytes and type 2 astrocytes are believed to arise from bipotential (O‐2A) glial progenitors. Previously we found that conditioned medium (CM) from the B 104 rat CNS neuronal cell line promotes growth of neonatal rat O‐2A progenitors in scrum‐free culture conditions with subsequent increases in differentiated progeny. We now report that O‐2A progenitors are present in mature rat brains and that this CM promotes the growth, motility, and bipolar morphology of these cells from 30‐ and 65‐day‐old rat brains, as shown by quantitative studies using double immunostaining and [^3^H]thymidine‐autoradiography. dition, the growth‐promoting action of B104 CM is not neutralized by antibodies to platelet‐derived growth factor, a proposed progenitor mitogen. Subsequent to the proliferation of these O‐2A progenitors, increases in oligodendrocytes and type 2 astrocytes occur. These data suggest a novel therapeutic strategy for some demyelinating diseases, e.g., multiple sclerosis, where there isa deficit in oligodendrocytes. Although it has been proposed by others thatmature brain O‐2A progenitors are less proliferative and thereby incapable of adequately replenishing lost oligodendrocytes in these diseases, we present in vitro evidence for continued response of mature brain O‐2A progenitors to this neuronal cell line‐derived mitogen.