A Spectrophotometric Method for the Dire
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Lisa A. Ridnour; Julia E. Sim; Michael A. Hayward; David A. Wink; Sean M. Martin
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Article
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2000
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Elsevier Science
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English
⚖ 117 KB
A method for the spectrophotometric determination of nitric oxide, nitrite, and nitrate in tissue culture media is presented. The method is based on the nitric oxide-mediated nitrosative modification of sulfanilic acid that reacts with N-(1-naphthyl)ethylenediamine dihydrochloride forming an orange-