Numerical taxonomy of Monascus species based on the electrophoretic analysis of intracellular enzymes

✍ Scribed by Jiro Nishikawa; Hiroshi Iizuka

• Publisher: John Wiley and Sons
• Year: 1993
• Tongue: English
• Weight: 700 KB
• Volume: 33
• Category: Article
• ISSN: 0233-111X
• DOI: 10.1002/jobm.3620330511

✦ Synopsis

Abstract

Four isozymes in fifteeen species of Monascus were analyzed using polyacrylamide gel electrophoresis. A numerical analysis was carried out on the basis of similarity values obtained from the electrophoretic mobilities of the enzymes. Non‐specific esterase and lactate dehydrogenase showed clearly distinguishable mobilities amont two clusters. From these results obtained, the strains used were divided into two clusters of Group‐I and Group‐II. Both groups were further subdivided into two subclusters. The type strain, M. ruber VAN TIEGHEM MR MO‐10 (IAM 8009) and M. kaoliang MR MO‐F2 were found to belong to the Group‐I. M. pilosus SATO MR MO‐4 (IAM8003) and M. pubigerus SATO MR MO‐8 (IAM 8004) were found to belong to the Group‐II. Nearly 100% similarity values were found between M. anka SATO MR MO‐6 (IAM 8001) and M. rubiginosus SATO IFO 4484, between M. anka var. rubellus SATO MR MO‐5 (IAM 8081) and M. purpureus WENT MR MO‐9 (IAM 8010), and, moreover, among M. vitreus SATO MR MO‐3 (IAM 8007), M. fuliginosus SATO IFO 4483 and M. serorubescens SATO 4487, respectively.

Therefore, it was concluded that electrophoretic analysis of enzymes may be useful in presenting a new classification system of Monascus spp.