Numerical and structural chromosomal abnormalities detected in human sperm with a combination of multicolor FISH assays
✍ Scribed by A. Baumgartner; P. Van Hummelen; X.R. Lowe; I.-D. Adler; A.J. Wyrobek
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 98 KB
- Volume
- 33
- Category
- Article
- ISSN
- 0893-6692
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✦ Synopsis
A pair of multicolor FISH assays (X-Y-21 and A-M-16) was developed for human sperm to simultaneously measure sex ratios; aneuploidies involving chromosomes 1, 16, 21, X, and Y; meiotic diploidies; and structural aberrations involving chromosome 1p. Sex ratios in sperm were not significantly different from unity among healthy men. Baseline frequencies of disomic sperm for chromosomes 1, 8, and 21 were similar (6.7 per 10 4 sperm, 95% CI of 5.6 -8.1), suggesting that among these three chromosomes, chromosome 21 was not especially prone to nondisjunction. Frequencies of disomy 16 sperm were significantly lower, however (3.5 per 10 4 sperm, 95% CI of 2.0 -6.2; P Ͻ 0.02). The baseline frequencies of sperm disomy by FISH for chromosomes 16 and 21 were validated against aneuploidy data obtained by the hamster-egg technique for human sperm cytogenetics. The frequencies of X-X, Y-Y, X-Y ("Klinefelter") sperm and sex-null ("Turner") sperm were 5.5, 5.1, 5.5, and 7.8 per 10 4 sperm, respectively. For chromosomes 16 and 21, the frequencies of nullisomic and disomic sperm were similar, suggesting that gain and loss events occurred symmetrically. However, more gain than loss was reported for chromosomes 1, X, and Y. The frequency of MI and MII diploid sperm (with flagella) was ϳ12 per 10 4 (range 8.3-16.7 per 10 4 sperm). Based on flagella data, the frequency of somatic cells in the semen was estimated to be ϳ1.8 per 10 4 sperm. Loss or gain of a portion of chromosome-arm 1p occurred in 5.5 per 10 4 sperm, and the percentage of sperm carrying structural aberrations within the haploid genome as calculated from FISH (1.4%), was similar to that obtained with the hamster-egg technique. These complementary sperm FISH assays have promising applications in studies of chromosomally abnormal sperm after exposure to occupational, medical, and environmental toxicants.