Nucleotide sequences of nuclear tRNACysgenes fromNicotianaandArabidopsisandexpressionin HeLa cell extract

✍ Scribed by Carsten Urban; Kathleen N. Smith; Hildburg Beier

• Book ID: 104615169
• Publisher: Springer
• Year: 1996
• Tongue: English
• Weight: 347 KB
• Volume: 32
• Category: Article
• ISSN: 0167-4412
• DOI: 10.1007/bf00019108

✦ Synopsis

Cys

We have recently characterized Nicotiana cytoplasmic (cyt) tRNAc¢ A as a novel UGA suppressor tRNA. Here we have isolated its corresponding (NtC 1) and a variant (NtC2) gene from a genomic library of Nicotiana rustica. Both tRNA Cy~ genes are efficiently transcribed in HeLa cell nuclear extract and yield mature cyt tRNAsCYL Sequence analysis of the upstream region of the RAD51 single-copy gene of the Arabidopsis thaliana genome revealed a cluster of three tRNA Cy~ genes which have the same polarity and comprise highly similar flanking sequences. Of the three Arabidopsis tRNA cy~ genes only one (i.e. AtC2) appears to code for a functional gene which exhibits an almost identical nucleotide sequence to NtC 1. These are the first sequenced nuclear tDNAs Cys of plant origin•