Nucleotide sequence of thepetA(cytochromef) gene from the green alga,Chlamydomonas reinhardtii

✍ Scribed by Jonathon Bertsch; Richard Malkin

• Book ID: 104616812
• Publisher: Springer
• Year: 1991
• Tongue: English
• Weight: 207 KB
• Volume: 17
• Category: Article
• ISSN: 0167-4412
• DOI: 10.1007/bf00036814

✦ Synopsis

A common feature of photosynthetic auxotrophs is the coupling of electron to proton translocation across a membrane. In green algae and higher plants this is mediated by the cytochrome b 6 f complex, which acts as a plastoquinol-plastocyanin oxidoreductas¢ transfers electrons between photosystems II and I [1 ]. Cytochrome b 6 f complexes consist of four (or possibly more) subunits, cytochrome b containing two hemes, cytochrome f, the Rieske Fe-S protein and subunit IV .

Recent work has been directed towards an understanding of both the function and assembly of this complex. In order to further study these questions at a molecular level, we are currently attempting to clone the genes for the subunits of the complex from Chlamydomonas reinhardtii.

In this report we present the sequence of the cytochrome f gene (petA) from C. reinhardtii (Fig. ). The gene was cloned from the Bam HI 7