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Nucleotide sequence of the gene for pre-apocytochromefin the spinach plastid chromosome

✍ Scribed by Juliane Alt; Reinhold G. Herrmann

Publisher: Springer-Verlag
Year: 1984
Tongue: English
Weight: 660 KB
Volume: 8
Category: Article
ISSN: 0172-8083
DOI: 10.1007/bf00410443

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✦ Synopsis

We have characterized a cloned fragment of the spinach plastid chromosome encoding the gene for apocytochrome f. Northern blot analysis and hybrid selection translation discloses that the gene is expressed. From the nucleotide sequence, we deduce that the protein contains 285 amino acids and an amino-terminal signal sequence of 35 amino acid residues. The calculated molecular mass of pre-apocytochrome f is 35.3 kd. The clustering of hydrophobic residues indicates that the processed protein (31.3 kd) possesses only a single anchoring transmembrane domain close to the C terminus, and that 75% of the polypeptide chain including the heme-binding site protrudes into the thylakoid lumen. This topology resembles that reported for beef heart mitochondrial cytochrome c1.

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