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Nucleotide sequence of the 5′-terminal region of rat 18S ribosomal DNA

✍ Scribed by Torczynski, Richard ;Bollon, Arthur P. ;Fuke, Motohiro

Publisher
Springer
Year
1983
Tongue
English
Weight
439 KB
Volume
191
Category
Article
ISSN
0026-8925

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✦ Synopsis

The 5'-terminal 597 base-pairs (bp) of the Sprague-Dawley rat 18S ribosomal RNA gene and 10 bp of the adjoining transcribed spacer have been sequenced. Previously sequenced 10 large oligonucleotides of rat 18S RNA were located in this region. This mammalian sequence has been compared with the known sequences of yeast and frog 18S rDNA's. The analysis indicates that 534 bp of the 597 bp (89%) are conserved between rat and frog sequences but only 75% of the nucleotides are conserved between rat and yeast in this region. Two large and two small sections have been identified where insertions have been introduced during evolution. Of these 58 bp long inserted sections of the rat rDNA sequence, 50 bp (86%) were G-C base-pairs.

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