✦ LIBER ✦

Nucleotide sequence of putC, the regulatory region for the put regulon of Escherichia coli K12

✍ Scribed by Nakao, Toshifumi ;Yamato, Ichiro ;Anraku, Yasuhiro

Publisher: Springer
Year: 1987
Tongue: English
Weight: 525 KB
Volume: 210
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00325707

No coin nor oath required. For personal study only.

✦ Synopsis

The nucleotide sequence of the putC region, from which divergent transcription of the putP and putA genes starts, was determined. The promoter region for the putA gene was restricted to the location between the HindIII site and the NcoI site or at the NcoI site by using putA-lacZ fusion plasmids and the transcriptional start for the putA gene was identified in the region between the HindIII site and the NcoI site by Sl mapping. This region also contains a potential CAP binding site, a ribosome binding site, and a sequence that is highly homologous to argTr. Five potential promoters (putPp1-putPp5) for the putP gene, which were separate from the promoter region for the putA gene, were indicated by S1 mapping analysis of the putP gene transcripts. We concluded that the putC region is 419 bp long and contains two independent sets of promoters, regulating the expression of putP and putA genes in opposite directions. In addition, this region was found to contain an open reading frame (orf) capable of encoding a polypeptide of 111 amino acids in overlapping fashion. But studies using an orf-lacZ fusion gene showed that this open reading frame was not expressed.

📜 SIMILAR VOLUMES

Nucleotide sequence of a regulatory regi

Nucleotide sequence of a regulatory region of the uidA gene in Escherichia coli K12

✍ Blanco, Carlos ;Ritzenthaler, Paul ;Mata-Gilsinger, Mireille 📂 Article 📅 1985 🏛 Springer 🌐 English ⚖ 471 KB

Nucleotide sequence of the hemB gene of

Nucleotide sequence of the hemB gene of Escherichia coli K12

✍ Echelard, Yann ;Dymetryszyn, Julien ;Drolet, Marc ;Sasarman, A. 📂 Article 📅 1988 🏛 Springer 🌐 English ⚖ 811 KB

The hemB gene of Escherichia coli K12, coding for porphobilinogen synthase (PBG-S; syn., 5-aminolevulinic acid dehydratase, ALA-D), was cloned following insertion of an EcoRI fragment of plasmid F'13 into the mobilizable vector pCR1. The hybrid plasmid carrying the hemB gene was able to complement a

Nucleotide sequence of the glnA control

Nucleotide sequence of the glnA control region of Escherichia coli

✍ Covarrubias, Alejandra A. ;Bastarrachea, Fernando 📂 Article 📅 1983 🏛 Springer 🌐 English ⚖ 564 KB

The RNA polymerase binding sites present along a DNA segment encompassing the glnA, glnL, and glnG genes have been identified in a hybrid plasmid carrying this chromosomal region of Escherichia coli. The DNA sequence was determined of an 817 base pair segment that contains the region coding for the

Isolation and nucleotide sequence of the

Isolation and nucleotide sequence of the hemA gene of Escherichia coli K12

✍ Drolet, Marc ;Péloquin, Luc ;Echelard, Yann ;Cousineau, Linda ;Sasarman, A. 📂 Article 📅 1989 🏛 Springer 🌐 English ⚖ 614 KB

The hemA gene of Escherichia coli K12 was cloned by complementation of a hemA mutant of this organism. Subcloning of the initial 6.0 kb HindIII fragment allowed the isolation of a 1.5 kb NheI-AvaI fragment which retained the ability to complement the hemA mutant. DNA sequencing by the dideoxy chain

Nucleotide sequence of putP, the proline

Nucleotide sequence of putP, the proline carrier gene of Escherichia coli K12

✍ Nakao, Toshifumi ;Yamato, Ichiro ;Anraku, Yasuhiro 📂 Article 📅 1987 🏛 Springer 🌐 English ⚖ 631 KB

The nucleotide sequence of the putP gene coding for the proline carrier in Escherichia coli has been determined and the amino acid sequence of the proline carrier deduced from it. The proline carrier is predicted to consist of 502 amino acids, resulting in a molecular weight of 54,343. The predicted

Nucleotide sequence and analysis of the

Nucleotide sequence and analysis of the mgl operon of Escherichia coli K12

✍ Hogg, Robert W. ;Voelker, Carolyn ;Von Carlowitz, Ira 📂 Article 📅 1991 🏛 Springer 🌐 English ⚖ 919 KB

The nucleotide sequence of the Escherichia coli K12 beta-methylgalactoside transport operon, mgl, was determined. Primer extension analysis indicated that the synthesis of mRNA initiates at guanine residue 145 of the determined sequence. The operon contains three open reading frames (ORF). The opera