Nucleotide sequence of pea seed-borne mosaic potyvirus coat protein gene

✍ Scribed by Elisabeth Johansen; Ole F. Rasmussen; Morten Heide; Bernhard Borkhardt

• Publisher: Springer
• Year: 1991
• Tongue: English
• Weight: 306 KB
• Volume: 5
• Category: Article
• ISSN: 0920-8569
• DOI: 10.1007/bf00271536

✦ Synopsis

cDNA of pea seed-borne mosaic potyvirus (PSbMV) RNA was synthesized and cloned in E. coli. Four overlapping clones that cover the complete PSbMV genome, except the extreme 5' terminus, were identified by restriction enzyme mapping, hybridization analysis, and partial sequencing. Overlapping cDNA clones covering 1386 nucleotides of the 3' terminus were sequenced. The nucleotide sequence contains one open reading frame (ORF), followed by an untranslated region of 163 nucleotides and a poly(A)-tract. The deduced amino acid sequence was found to include the C-terminus of the predicted RNA-dependent RNA polymerase and the coat protein. A glutamine-alanine dipeptide was identified as a putative 49-kD proteinase cleavage site at the N-terminus of the coat protein.