Nucleotide sequence of katG of Salmonella typhimurium LT2 and characterization of its product, hydroperoxidase I
โ Scribed by Loewen, PeterC. ;Stauffer, GeorgeV.
- Book ID
- 104688170
- Publisher
- Springer
- Year
- 1990
- Tongue
- English
- Weight
- 539 KB
- Volume
- 224
- Category
- Article
- ISSN
- 0026-8925
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โฆ Synopsis
The nucleotide sequence of katG from Salmonella typhimurium was determined revealing an open reading frame of 2181 bp that could encode a 727 amino acid protein. The predicted sequence of the encoded hydroperoxidase I (HPI) was found to be 90% similar to HPI from Escherichia coli and was one amino acid longer. The physical and enzymatic properties of HPI from both Salmonella typhimurium and Escherichia coli were found to be virtually identical despite the 10% divergence in sequence.
๐ SIMILAR VOLUMES
We have sequenced the fruR gene and flanking DNA fragments from Escherichia coli K12 and Salmonella typhimurium LT2. The fruR gene codes for a protein that represses the fru operon and activates the pps gene for PEP synthase. The corresponding open reading frame (ORF) FruR consists of 334 amino acid