Nucleotide sequence of a new class A β-lactamase gene from the chromosome of Yersinia enterocolitica: Implications for the evolution of class A β-lactamases

✍ Scribed by Seoane, Asunción ;García Lobo, Juan M.

• Publisher: Springer
• Year: 1991
• Tongue: English
• Weight: 642 KB
• Volume: 228
• Category: Article
• ISSN: 0026-8925
• DOI: 10.1007/bf00282468

✦ Synopsis

The nucleotide sequence has been determined of a 1400 bp fragment from the chromosome of Yersinia enterocolitica containing the gene for beta-lactamase I. An ORF of 882 bp was identified, which could code for a polypeptide of 294 amino acids, closely related to other beta-lactamases of molecular class A. Amino acids 1-30 could constitute a signal peptide. The mature protein would be 264 amino acids long with a calculated pI of 6.2. Alignment of the amino acid sequence of the class A beta-lactamases suggested the existence of two subgroups in the same class, and this is discussed in the context of the evolution of the enzymes.