Nucleotide sequence of a mouseTcp-1pseudogene: A nucleotide record for atcomplex gene carried by an ancestor of the mouse

✍ Scribed by Hiroshi Kubota; Takashi Morita; Yoko Satta; Masami Nozaki; Aizo Matsushiro

• Book ID: 104737485
• Publisher: Springer-Verlag
• Year: 1992
• Tongue: English
• Weight: 671 KB
• Volume: 2
• Category: Article
• ISSN: 0938-8990
• DOI: 10.1007/bf00355434

✦ Synopsis

We have isolated clones of a processed pseudogene of mouse t complex polypeptide 1 (Tcp-1) and determined the nucleotide sequence of the pseudogene. The pseudogene was 1363 bp long and had no intron. The Tcp-1 pseudogene had 88.4% or 88.3% nucleotide identity to the mouse Tcp-1 cDNA of wild-type (Tcp-1 b) or t haplotype (Tcp-U), and 87.5% identity to the rat Tcp-1 cDNA. On 12 nucleotide positions where the open reading frames (ORFs) of mouse Tcp-1 b and Tcp-U cDNAs have bp substitutions, the Tcp-1 pseudogene had 6 bp identical to Tcp-1 b, 5 bp identical to Tcp-U and 1 bp not identical to neither. On ten amino acid positions where TCP-1B and TCP-1A polypeptides have substitutions, deduced amino acids of the Tcp-1 pseudogene had four amino acids identical to TCP-1B, five amino acids identical to TCP-1A and one amino acid identical to neither. These results suggest that the ancestral mouse Tcp-1 gene would have had no significant difference between the resemblance to Tcp-t ~ and that to Tcp-I ~ before they were diverged and that amino acids of TCP-1B and TCP-1A would have been substituted in similar high rates.