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Nucleotide sequence of a genomic gene encoding tritin, a ribosome-inactivating protein fromTriticum aestivum

✍ Scribed by Noriyuki Habuka; Jiro Kataoka; Masashi Miyano; Hideaki Tsuge; Hideo Ago; Masana Noma

Publisher
Springer
Year
1993
Tongue
English
Weight
512 KB
Volume
22
Category
Article
ISSN
0167-4412

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✦ Synopsis

A genomic gene of tritin, a ribosome-inactivating protein (RIP) from Triticum aestivum, was cloned using a barley RIP gene as a probe. The 5'-non-coding region has potential TATA boxes and three sequences homologous to the binding sequence of the transcriptional activator protein Opaque-2 which activates maize RIP gene expression. The cloned DNA encoded tritin consists of 275 amino acids with no secretion signal sequence. The coding region of tritin was expressed in Escherichia coli using lac promoter and yielded a protein similar to the native one, as determined by SDS-polyacrulamide gel electrophoresis and immunological analysis.

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