๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Nucleotide sequence-based typing of bacteria and the impact of automation

โœ Scribed by Stuart C. Clarke

Publisher
John Wiley and Sons
Year
2002
Tongue
English
Weight
121 KB
Volume
24
Category
Article
ISSN
0265-9247

No coin nor oath required. For personal study only.

โœฆ Synopsis

DNA-based typing methods are increasingly important for the characterisation of bacteria. They are used to monitor the epidemiology of pathogens with public health significance and also to help understand the evolution and population biology of bacteria. However, these methods require accuracy and reproducibility and are often of a high-throughput nature. Laboratory automation is therefore the key to the successful implementation of such methods. This review describes the impact of automation on DNA-based typing methods, particularly multi-locus sequence typing (MLST), and the method components that can be automated.

๐Ÿ“œ SIMILAR VOLUMES

Protein purification and nucleotide sequ
Protein purification and nucleotide sequence of a lysozyme from the bacteria-induced larvae of the fall webworm, Hyphantria cunea
โœ Ho-Yong Park; Soon Sik Park; Sang Woon Shin; Doo-Sang Park; Mi Gwang Kim; Hyun W ๐Ÿ“‚ Article ๐Ÿ“… 1997 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 253 KB ๐Ÿ‘ 2 views

A protein with lytic activity against Micrococcus luteus was purified from the hemolymph of the fall webworm, Hyphantria cunea, larvae challenged with live E. coli. A bacteriolytic protein of about 14,000 daltons in mass was purified by cation exchange chromatography and reverse-phased HPLC. The opt

Dependence of the Raman signature of gen
Dependence of the Raman signature of genomic B-DNA on nucleotide base sequence
โœ Hong Deng; Victor A. Bloomfield; James M. Benevides; George J. Thomas Jr. ๐Ÿ“‚ Article ๐Ÿ“… 1999 ๐Ÿ› Wiley (John Wiley & Sons) ๐ŸŒ English โš– 191 KB ๐Ÿ‘ 2 views

The vibrational spectra of four genomic and two synthetic DNAs, encompassing a wide range in base composition [poly(dA-dT) 72% G ฯฉ C; poly(dG-dC)โ…poly(dG-dC), 100% G ฯฉ C] (dA: deoxyadenosine; dG: deoxyguanosine; dC: deoxycytidine; dT: thymidine), have been analyzed using Raman difference methods of

The maximum information principle and th
The maximum information principle and the evolution of nucleotide sequences
โœ Liaofu Luo; Guoyi Bai ๐Ÿ“‚ Article ๐Ÿ“… 1995 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 356 KB

The probability distributions of bases in nucleotide sequences are deduced from the maximum information principle by maximizing the entropy (due to random mutation of bases) under certain constraints (Markovian entropy, G+C content, etc, due to selection). Two formulations are given with respect to