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Nucleotide sequence and molecular characterization of a maize mitochondrial plasmid-like DNA

✍ Scribed by Alan G. Smith; Daryl R. Pring

Publisher
Springer-Verlag
Year
1987
Tongue
English
Weight
946 KB
Volume
12
Category
Article
ISSN
0172-8083

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✦ Synopsis

The mitochondrial genome of Black Mexican Sweet maize consists of the principal genome, a 2.3 kb minilinear DNA, a 1,913 bp (1.9 kb) and a 1,445 bp (1.4 kb) minicircular DNA. The three extrachromosomal DNAs exhibit characteristics of autonomous replication in cell suspension culture. The complete sequence of the 1.4 kb minicircle was determined. It has 61 bp of near perfect sequence homology to the 1.9 kb minicircle. Both minicircular DNAs are transcriptionally active; the longest open reading frame of the 1.4 kb minicircle was 231 bp. A putative origin of replication was identified as a high A+T sequence. These minicircles were present in some but not all of 20 maize lines surveyed. None of the lines examined carried the 1.4 kb minicircle without the 1.9 kb minicircle. Nuclear DNA of one line of the seven examined carried homology to both DNAs.

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Cloning and characterization of a linear
Cloning and characterization of a linear 2.3 kb mitochondrial plasmid of maize
✍ Bedinger, Patricia ;de Hostos, Eugenio L. ;Leon, Patricia ;Walbot, Virginia πŸ“‚ Article πŸ“… 1986 πŸ› Springer 🌐 English βš– 890 KB

A linear 2.3 kb DNA molecule found in maize mitochondria was cloned into pUC8. A natural deletion of this plasmid, found in cmsT and some N (fertile) types of maize plants, was mapped to one end of the plasmid. A minor sequence homology to S-2, another linear mitochondrial plasmid, was detected, as