Nucleotide sequence and expression of the gene for the 9 kDa FA/FBcomponent of Photosystem I from the cyanobacteriumAnabaenasp. PCC7120
β Scribed by Martin E. Mulligan; Donna M. Jackman
- Publisher
- Springer
- Year
- 1992
- Tongue
- English
- Weight
- 503 KB
- Volume
- 18
- Category
- Article
- ISSN
- 0167-4412
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β¦ Synopsis
The nucleotide sequence of an 813 bp Ssp I-Hinc II fragment containing the psaC gene from Anabaena sp. PCC 7120 is reported. The gene encodes a polypeptide of 81 amino acids, has a single transcript of size approximately 480 nucleotides and a single startpoint of transcription. It is flanked by a ribosome binding site on the 5' end and a potential transcription terminator on the 3' end. Comparison of the amino acid sequences of the psaC gene product from sixteen organisms shows that cyanobacteria group as a cluster distinct from monocotyledonous and dicotyledonous plants and that individual amino acids are diagnostic of each cluster.
π SIMILAR VOLUMES
Several cDNA clones encoding the complete subunit II and III precursor polypeptides of the photosystem I reaction center were isolated from two spinach lambda gt1 1 expression libraries by immunoscreening and homologous hybridization. The identity of the recombinant cDNAs was verified by an N-termin